![]() ![]() Users are expected to provide their own SEC columns.Users are expected to provide all consumables specific to their experiment.For detailed information please contact Dr. ![]() Regular user groups are expected to opt into the pool and pay an monthly share that goes towards the general up keep of the Shared Instruments Pool.In CG-MALS a composition-gradient preparative system is combined with MALS/RI/UV detectors to measure interactions between biological macromolecules, by directly observing changes in molar mass due to complex formation or dissociation in solution and without the need for labeling or immobilization. In contrast to conventional SEC chromatography this method can accurately measure MW, even for sticky or elongated proteins that elute to late or too early for their actual mass to be correctly determined by size exclusion chromatography SEC alone (due to their unusual interaction with the columns).Īn integrated dynamic light scattering detector allows measurement of the hydrodynamic radius (R h) of particles, with a lower limit of 0.5 nm. SEC-MALS can be used to determine the absolute molecular mass of proteins and complexes in complex samples, independent of their elution volume from the SEC column. SEC-MALS uses size exclusion chromatography (SEC) coupled to an in-line multi- angle light scattering detector and an in-line refractive index or UV/Vis detector. MALS in conjunction with a concentration detector can directly measure the absolute molar masses of macromolecules and complexes, independent of structure and shape from 200 daltons to hundreds of millions of daltons. ![]()
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